Dire Wolves disappeared about 12,500 years ago. Now, thanks to genetic engineering, they have returned to what scientists described as “the first extraction of the world”.
Scientists in Colossal Biosciences, a biotechnology -based protection company Bring the woolen mammoths backTell them that they produce three terrible wolves (Aenocyon Dirus) puppies. They did this by rebuilding the wolf’s genome DNADefining the versions of genes seen in terrible wolves, not only in living relatives of extinct carnivores.
The team then DNA is from modern gray wolves (Canis Lupus) To include this unique gene variants. They placed DNA more gray wolves in egg cells and allowed the proxy dogs to mature in the laboratory before entering their wombs, Notified time. Three embryos, Romulus, Remus and Khaleesi, who successfully implanted in the three -pro -dog dogs, lead to the births of snowy white puppies, reminiscent of ghosts, Jon Snow’s face wolf. “game of Thrones“TV series.
Relating to: ‘We didn’t know they would be so cute’: scientists ‘woolen mice designed genetically’
“Our team took DNA from a 13,000 -year -old tooth and a 72,000 -year -old skull and made a healthy terrible wolf offspring.” He said. “Once upon a time ‘enough advanced technology cannot be distinguished from magic.’ Today, our team will explain the spell they are working on.
How to make a terrible wolf
Terrible wolf team for “-Sering”, Dolly sheep In 1996.
With this method, a single cell is removed from the cloning subject to produce a perfect clone and the genetic material held in the nucleus of the cell is removed. This genetic information is then inserted into the egg of a donor of the same species whose nucleus is removed. The egg is now containing all genetic information to reconstruct the original cloning issue and is transferred to a proxy for the period.
However, Dire Wolves had to approach his own terrible wolf cells by bringing tips from the huge, fossil records, as he was extinct at the end of the last ice age.
To rebuild the animal’s genome, they used genetic examples of terrible wolf fossils and compared them to the genomes of their live relatives, including wolves, jackals and foxes. They chose gray wolves to become egg donors, because these animals are terrible cousins.
This genomic analysis enabled scientists to identify 20 main differences in 14 genes responsible for their distinctive features, including larger dimensions, white coats, larger teeth, and characteristics of the terrible wolves.
Later, they gathered cells from the blood samples of live gray wolves – a method less than using a texture for Dolly The Sheep. Then he uses CRISPR The team, which is a gene editing technology, made 20 arrangements described in 14 again, so that they were in the same order as Wolves’s former relatives.
After these “terrible wolf cells” were created, their seeds were removed and inserted into the eggs from a living gray wolf. As in Dolly Koyun, these donor egg cells have been removed from their nuclei to make room for the new DNA. Reproductive cells – such as eggs and sperm – normally has only one copy of each chromosome; However, the cells in the body carry two copies. Therefore, the transfer of the nucleus to a reproductive cell from a body cell provides all genetic instructions for ripening into an embryo.
Eggs were allowed to mature embryos in the laboratory, which resulted in 45 embryos transferred to the uterus of two pets, a sub -type of gray wolf.
Only one embryo was kept in each attorney. After 65 days of pregnancy, Romulus and Remus were born on October 1, 2024 through the cesarean section. A few months later, the whole process was repeated with a third -proxy dog that gave birth to Khaleesi three months later.
He shared the images of Romulus and Remus as he took their first steps, making their first ulums and developing young puppies from newborns. They described terrible wolves as “the world’s first removal.”
However, there is another compensation: In 2003, scientists in Spain, a descended brutal goat species known as Bucardo or a Pyrenean IBEX (Capra Pyrenaica Pyrenaica). However, the newborn goat died just a few minutes after birth, 2009.
The company said in a statement that two -liter cloned red wolves, the most critical wolf in the world, was born.
The announcement comes only months after the gigantic introduction Genetically designed “woolen mice”It is a fur similar to the thick hair of woolen mammoths. Back these ice age titans until 2028.
